羊仰口線蟲PCR檢測試劑盒操作流程: 1.從室溫平衡20min后的鋁箔袋中取出所需板條,剩余板條用自封袋密封放回4℃。 2.設置標準品孔和樣本孔,標準品孔各加不同濃度的標準品50μL; 3.樣本孔中加入待測樣本50μL;空白孔不加。 4.除空白孔外,標準品孔和樣本孔中每孔加入辣根過氧化物酶(HRP)標記的檢測抗體100μL,用封板膜封住反應孔,37℃水浴鍋或恒溫箱溫育60min。 5.棄去液體,吸水紙上拍干,每孔加滿洗滌液(350μL),靜置1min,甩去洗滌液,吸水紙上拍干,如此重復洗板5次(也可用洗板機洗板)。 6.每孔加入底物A、B各50μL,37℃避光孵育15min。產品僅用于科研實驗 7.每孔加入終止液50μL,15min內,在450nm波長處測定各孔的OD值。 小鼠糖皮質激素受體β(GR-β)ELISA KitMouse glucocorticoid receptor-β,GR-β ELISA Kit 小鼠糖皮質激素受體α(GR-α)ELISA KitMouse glucocorticoid receptor-α,GR-α ELISA Kit 小鼠糖類抗原199(CA199)ELISA KitMouse Carbonhydrate Antigen CA199 ELISA Kit 小鼠糖類抗原125(CA125)ELISA KitMouse Carbonhydrate Antigen 125,CA125 ELISA Kit 小鼠碳酸酐酶1(CA-1)ELISA KitMouse carbonic anhydrase 1,CA-1 ELISA Kit 小鼠胎球蛋白A(Fetuin A)ELISA KitMouse Fetuin A ELISA Kit 小鼠胎盤生長因子(PLGF)ELISA KitMouse placenta growth factor,PLGF ELISA kit 小鼠胎盤泌乳素(PL)ELISA KitMouse placetal lactogen,PL ELISA Kit 小鼠鎖鏈素(Desmosine)ELISA KitMouse Desmosine ELISA Kit 小鼠羧化不全骨鈣素(ucOC)ELISA KitMouse Undercarboxylated Osteocalcin,ucOC ELISA Kit 小鼠髓樣細胞觸發性受體2(TREM2)ELISA KitMouse Triggering receptor expressed on myeloid cells 2,TREM2 ELISA kit 小鼠髓樣分化因子88(MyD88)ELISA KitMouse myeloid differentiation factor 88,MyD88 ELISA Kit 小鼠髓過氧化物酶特異性抗中性粒細胞胞質抗體(MPO-ANCA)ELISA KitMouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit 小鼠酸性磷酸酶(ACP)ELISA KitMouse Acid Phosphatase,ACP ELISA Kit 小鼠酸性成纖維細胞生長因子(aFGF/FGF-1)ELISA KitMouse acidic fibroblast growth factor,aFGF/FGF-1 ELISA Kit 小鼠酸性β葡萄糖苷酶(GBA)ELISA KitMouse glucosidase, beta; acid (includes glucosylceramidase) (GBA)ELISA kit 小鼠絲裂原激活的蛋白激酶/MAP激酶(Mapk1/Erk2/Mapk/Prkm1)ELISA KitMouse mitogen-activated protein kinase 1 ELISA kit
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